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Total Motile Count

tillietillie Member Member
Thought it might be nice to start a thread where people could report in on vials of donors recently used and what the TMC and motility % was so that others may know which donors are looking potent and which they may want to consider avoiding. If you've had an IUI (or ICI) recently, what were your donor's numbers pre and post wash?

Comments

  • manda210manda210 Member Member
    We used 4034 last month for IUI and I was told it was 55 million I'm guessing post wash since thats how it was sent to the doctor...my doctor said it was a GREAT number and from what I read it is BUT it didnt work =( hopefully next time it will tho
  • karlip79karlip79 Junior Member
    Just did my first IUI with donor 2973 His total motile count was 10.1 million motile after washing. I bought the unwashed (ICI) vial, because my doctor likes to do the washing process himself in his office. This is a newer donor with no reported pregnancies, but I am hoping to change that after my (grueling) 2 week wait! Good luck to all og you (:
  • maria2maria2 Senior Member Senior Member
    I used donor #2959 - purchased ICI (unwashed) b/c my doctor's office washes...post-wash it was 50+ million with 37% motility. Doctor told me that its' a bit slow but not to worry about it. I'm going to get inseminated again soon with donor #2959 (yes, again)....hopefully this time it will work. I'll email with #s later.
  • Two2148SonsTwo2148Sons Junior Member Junior Member
    I just want to caution that the counts in the vials aren't always indicative of whether you will succeed. I used the same donor for both of my children. With the vial that I conceived my first son, the RE clinic said it was one of the best samples they had seen of a thawed sample. With the vial that I conceived my second son, the RE clinic said the count/motility was pretty low and said I might even qualify for my $ back or a replacement. It also ended up being an ICI due to the nurse not being able to get the catheter in. I was so bummed out. I figured bad sample+ICI = zero chance. Well, I conceived that cycle (even had a positive HPT at 8DPO). I have two perfect little boys, one from a great sample, one from an iffy sample!
  • tillietillie Member Member
    Is there a question as to what these counts really mean because doctors use different methodologies and people throw around the no. of millions but the number may apply to what is in the vial or other drs. may give the millions that are just what is in the smaller amount inserted in the IUI? I ask this because a friend told me that someone at Fairfax said that it would be unheard of for them to have an ICI or IUI vile that has 50 million TMC. That makes sense that they wouldn't be selling a vial of (or is it ML) 50 million to some and 10 million to others. Fairfax seemed to suggest that most commonly their vials are just over their minimums.

    Does anyone know more about this? Are all doctors using the numbers to refer to the same thing?
  • maria2maria2 Senior Member Senior Member
    Ok...so I was confused. My #s were pre-wash for #2959. I had my 2nd iui and his numbers pre-wash were pretty much same...51 million with 37% motility. Now on to 2ww....
  • Fairfax CryobankFairfax Cryobank Administrator Senior Member
    Here is some information about Fairfax Cryobank's specimen quality standard:
    Sperm counts will vary 10-30% depending on the lab personnel and counting method. Laboratory variation is expected and taken into consideration when processing complaints. The possibility exists that sperm counts performed at other laboratories may be less than the stated specimen standards. Semen is not a homogenous or uniform mixture which adds to sample variability. A variation in count does not indicate the specimen is not suitable for insemination or that a pregnancy will not result if used for an insemination. Fairfax Cryobank takes these variations into account when determining eligibility for refund or credit.

    Please visit our website to read more details about specimen quality: http://www.fairfaxcryobank.com/qualitystandard.shtml

    Also, check out this interesting blog post from Michelle Ottey, PhD, Fairfax Cryobank Director of Operations: How much sperm is enough?
  • tillietillie Member Member
    For those posting big numbers, like 55 million, are those the "concentration" or the "total motile sperm." My specimen report for each IUI has volume, concentration, motility %, progression, and total motile sperm. The concentration numbers are from 50 million to 130 million, but the numbers that I am concerned with and which matter most are the total motile sperm. Pre-wash, my total motile sperm numbers have ranged from 27 million to 10 million and my post-wash numbers are obviously smaller for total motile sperm. My progression numbers and motility %s haven't been super.

    I think often times we have numbers that are given to us or that we see but we are not talking apples to apples.
  • manda210manda210 Member Member
    Used 4034 again today for our IUI...his total sperm count postwash was 58 million this time and i checked the mobility this time and it was 35%....now the 2ww...i hope that it works this time
  • tillietillie Member Member
    Did you get the total motile count number post wash instead of the total sperm count? Apparently, the most important number is the total motile sperm count post wash, which should range from 3-5 million (on the way low side) up to something like 10 or 20 million. Maybe it gets a bit higher but I don't think a TMC will be much higher than that. The 35% is what takes the 55 million of the total sperm down - they do some complex calculation using those numbers and the amount etc.
  • tillietillie Member Member
    manda210 wrote:
    Used 4034 again today for our IUI...his total sperm count postwash was 58 million this time and i checked the mobility this time and it was 35%....now the 2ww...i hope that it works this time

    Oh and good luck!
  • manda210manda210 Member Member
    Im not sure I just read the paperwork that came with the sperm to the doctors office it said 58 million and 35 percent
  • karlip79karlip79 Junior Member
    Used 2973 last month (10.1 million) unsuccussful, switching donors this month to 4008. 11 days til IUI. will keep you all posted as to the count of the new donor 4008. ;)
  • moewhitmoewhit Senior Member
    Just had my #3 IUI today I used donor 4187 his prewash=58 mil, postwash=38 mil with 42% motility. Hope it works this time:-)
  • karlip79karlip79 Junior Member
    Hi ladies, Went in today for my 2nd IUI (first was unsuccussful last month) and am actually a little dissappointed. Total motile count was only 4.6 million motile post wash today. ): This was significantly less than last time.( 10.1 million motile with a different donor) I do not blame fairfax, they have always been wonderful to deal with, and I understand these things happen and there are quality assurances in place, but of course it is a bummer, I did have 3 good mature follies, and I know it takes only 1 good swimmer so I am trying to be hopeful in spite of the number. The donor I used was 4008. I guess I will have to keep my fingers crossed and update you all in 2 weeks!
  • babynumber2babynumber2 Junior Member
    Hi ladies! I had my first IUI today and 4001 count was 13 million. The doctor said that was a really good number. It hasn't even been 12 hours since the procedure though and it already feels like 12 years--these next 2 weeks are going to be a killer!
  • sibemomsibemom Member Member
    Not sure how the count really equates... but as I am going to mention in another thread for the donor, 2976... I had my second attempt today. At the onset my doctor comes in and says "we need to talk" and she proceeded to say that looking at things under the scope there was barely any movement and she asked where I got it from. Kind of disheartening.
  • tillietillie Member Member
    Sorry to hear that. You should call your RE tomorrow or the nurse and ask for all the numbers for the vial(s) that you used. I'd get the total motile count pre-wash after the thaw and the total motile count post-wash. And the motility percentages pre- and post-wash. Then also get the concentration numbers for both and also the progression numbers for both. The progression is I think a rating (1-4) that they give the sperm for its movement. They may have a report they can copy for you that has all this info.

    Then report it all back to us and check the numbers against Fairfax's warranty to see if you need to have your RE call Fairfax ASAP to report the warranty issue.
  • sibemomsibemom Member Member
    It is IUI ready - already washed. I mean, with the small vial that comes - I can't imagine them using it for testing this that and the other. I have the numbers that Fairfax gave me when they put it into storage, which meets their 10mil threshold. I've read and read and this stuff all seems so foreign and I'd hope you wouldn't have to verify the experts, ya know?
  • tillietillie Member Member
    sibemom wrote:
    It is IUI ready - already washed. I mean, with the small vial that comes - I can't imagine them using it for testing this that and the other. I have the numbers that Fairfax gave me when they put it into storage, which meets their 10mil threshold. I've read and read and this stuff all seems so foreign and I'd hope you wouldn't have to verify the experts, ya know?

    Yeah, I would just confirm with your RE that indeed it met the 10 mill threshold. If it did, that should mean that those are the motile ones that are movers and you're ok. But I'm definitely no expert. I wonder if maybe some sperm just does not do well surviving the thaw. The 10 mill warranty means the 10 mill has to hold up post thaw.

    Sorry for the disappointment. I hope the RE was wrong. This stuff is confusing though. I do sometimes wonder if CCB does far better with the sperm quality of the vials they sell.
  • karlip79karlip79 Junior Member
    Hi there, just a quick response about the low count or motility. I purchased ICI vial from 4008. The number pre wash was only 6 and a half million , and went down to 4.6 post wash. Fairfax guarantees 10 million , and will issue a refund if a complaint is propperly filed by your dr.'s office within 10 days. I am confident the thawing was done properly, I live in Las Vegas and my IUI was performed by Dr. Sher who literally invented IUI in 1982. (I know because he reminds me every time I see him! lol) We still did the insemination, but obviously I am not letting myself get my hopes up too high. As for the mom considering California Cryo bank, My Dr. gets so many poor samples from them he literally recomends patients to look elsewhere. I believe bad samples can happen anywhere, but are fewer and further between here, plus, I know it is a bummer to waste a cycle, but the $ guarantee is in place to protect you and your wallet. We have decided to order 2 vials next time, because the nurse said if I had another sample available she could have combined the two, now my only dillema is weather to switch donors or not, since with the last sample even if I would have combined two like vials, I would still not have reached 10 million. Hmmmmm. Food for thought. any sugguestions? :?
  • jl2011jl2011 Member Member
    Hi...I too had a bad vial. My first IUI the count was only 3 million. I purchased the pre washed for IUI vial and it was still only 3 million. I didn't have to do anything. My doctor's office handled everything including all the paperwork and the everything included in the complaint process. After it didn't work and I got my period the doctor's office confirmed it to Fairfax and I got a refund no questions asked. I didn't get a refund on shipping though. I chose for them to just credit my account so I could make another order. It was easy and they were incredible to work with. I tried again with the same donor and the numbers were better but still only 10 million.
    I'm taking some time off. But, when I try again I will be using another donor. Good luck!
  • Fairfax CryobankFairfax Cryobank Administrator Senior Member
    From Fairfax Cryobank's Laboratories Manager:

    Hello. I reviewed this thread and thought it may be helpful to give you an explanation of how counts are completed and what numbers are important.

    Every donor specimen is analyzed for several parameters, the most relevant to this discussion are the count, motility, and progression. The specimen must meet our internal high standards in order to be processed.

    When a specimen is processed as an IUI pre-washed sample we do a wash to remove the seminal fluid then do a second analysis to ensure the quality is still within acceptable range. A freezing media with cryoprotectant is added to the sample, then the sample is checked again for quality, then frozen.

    Some of the sperm will not survive the process of being frozen and thawed. The cryoprotectant obviously serves to improve those numbers. We place a specific number of sperm cells in each vial to account for the freeze/thaw loss. Every specimen then has a test vial thawed to ensure a good post thaw motility and confirmation of the total motile cells after the freeze/thaw.

    Our quality standard states that there will be 10 million total motile cells in each vial for our ICI and IUI preps. If a specimen’s test vial does not meet this standard we will not sell the specimen date.

    We state in our Specimen Quality Standard, as do most Cryobanks, that there is counting variability between labs due to technician variability, counting chambers being used, etc. This means that we may count the sample and grade the motility and have a final TMC of 10 million, and your clinic may look at the sample and say they find 12.4 million TMC or 9 million TMC.

    If a pre-process analysis from your clinic demonstrates a TMC lower than our 10 million TMC standard and you do not achieve pregnancy the vial is eligible for a refund.

    It is important to look at the clinic’s pre-process analysis as any manipulation of a sample will alter the numbers. If the clinic does the analysis and determines a TMC of 10 million or more then does a wash on a sample and the second analysis shows 8 million TMC, that vial is not eligible for a refund.

    The analysis uses an incredibly small volume of the sample.

    I hope you find this helpful.
  • karlip79karlip79 Junior Member
    So, I am really torn as to what to do now. I had an IUI 1 week ago with the poor sample mentioned earlier in this thread, I just love this donor, and would like to use him again if pregnancy is not achieved this time, but I am wondering if one sample was poor quality from this particular donor, does that mean he is more likely to have more poor quality samples when compared to other donors, or is each poor sample an isolated incident? Any info or sugguestions?
  • Stellafaerie13Stellafaerie13 Junior Member Junior Member
    I realize this is an old thread, but I think it's a useful one. I just received donor 5104, with 10 million TMC but only 35% motility. Planning first IUI this month, natural cycle, home-monitored. Will try to remember to post results asap.
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